The reason I ask is that my beautiful Lilycat really seems to be more of a boy than a girl. I know she's physically a neutered female and when the vet removed her girl parts, she (the vet) didn't say anything about there being any anomalies, but she looks and acts like a big male.
Having had several transgender friends, I'm conscious of the gender identity issue for humans and maybe that makes me more alert to gender signals, but I have caught myself calling Lily "he" and "him", even though she has a female name and is a black tortoise-shell calico, a color pattern which is almost never male.
Lily is big and bulky, not fat, but she probably weighs 18 pounds and she behaves in a boyish way. I think I've become more conscious of this behavior since Maxie came to live with us. Maxie is an as-yet-tomkitten who is definitely full of testosterone, even at 5 months, though fortunately he hasn't displayed any spraying behaviors and will be off to the vet himself in a month or so for a little clipping of the nether regions.
I have read somewhere that sexual orientation is a feature of all animals, not just humans, and that homosexual behavior is displayed by a percentage of all animal life. Because Lily is spayed, she's not getting any sex hormones, so I can't say that she's a lesbian cat. But there is something different about Lily and it seems to be her masculine nature in a female body. Her nature is so different from Loosy's nature that it's remarkable, and some of the differences (not all) seem gender-ish in nature.
I used to think it was because she had a feral mama and might have absorbed some of her mama's wild instincts. Now I wonder if her nature may be more connected to her sense of being different.
Of the three cats, s/he is probably my favorite because she is so different. She is absolutely gorgeous but very wary of strangers and takes a long time to warm up to anyone else. Yet she will hoist her majestic bulk up into my lap, plant her paws against my bosom and her head into my chin, and purr till the house rattles. She is pretty much a one-person cat and this is very flattering, of course.
When Maxie came along, Lily was initially very wary of him, seeing him, no doubt, as a competitor for my affection. But she came to terms with him much sooner than Loosy, who still hisses and swats him angrily, and the two of them are more like big brother/little brother. Maxie seems to consider Lily his bud and he clearly considers Loosy a girl to tease and torment.
Now, I know I'm anthropomorphing my cats and that there may be no substance to what I'm theorizing. But I've been wondering about this for awhile. If anyone has any evidence or helpful input, I'd appreciate it. Her gender identity is not a problem of any kind, it's just interesting.